Journal: Cell Reports

Article Title: Cytokine responsive networks in human colonic epithelial organoids unveil a molecular classification of inflammatory bowel disease

doi: 10.1016/j.celrep.2022.111439

Figure Lengend Snippet: A gradient of cytokine-responsive transcriptional signatures stratifies patients with IBD into distinct molecular phenotypes (A) Activation of cytokine-responsive transcriptional signatures in whole colonic biopsies of patients with UC (UNIFI trial, n = 550) and colonic CD (UNITI2 trial, n = 126) (demographics shown in Table S1 ). Enrichment of cytokine-responsive transcriptional signatures was evaluated in the transcriptome of colonic biopsies using gene set variation analysis. A score of +1 suggests that all transcripts are upregulated, while a score of −1 suggests that all transcripts are downregulated (showing median and interquartile range [IQR], Mann Whitney test, ∗∗∗ p < 0.001). (B, C, and D) Gradient of cytokine-responsive transcriptional signature activation in IBD. Each column represents a single patient. The sum of all four scores per subject is also depicted as the total enrichment score (TES). Columns have been clustered by Euclidean distance (method: average, tree ordering: original, figure generated with ClustVis). Cohorts: UNITI2 (n = 126), UNIFI (UC, n = 550), and PROgECT (UC, n = 84). (E and F) Cytokine-responsive transcriptional signature association to clinical indices of human colonic inflammation in UC (UNIFI cohort, n = 550) and cCD (UNITI2 cohort, n = 126). All clinical data were collected prospectively as part of the trials’ protocol ( Feagan et al., 2016 ; Sands et al., 2019 ; Telesco et al., 2018 ).

Article Snippet: Human intestinal biopsies for whole transcriptome profiling (microarray) , Janssen Pharmaceuticals , UNITI2, NCT01369342 UNIFI, NCT02407236 PROgECT, NCT01988961.

Techniques: Activation Assay, MANN-WHITNEY, Generated

Journal: Cell Reports

Article Title: Cytokine responsive networks in human colonic epithelial organoids unveil a molecular classification of inflammatory bowel disease

doi: 10.1016/j.celrep.2022.111439

Figure Lengend Snippet:

Article Snippet: Human intestinal biopsies for whole transcriptome profiling (microarray) , Janssen Pharmaceuticals , UNITI2, NCT01369342 UNIFI, NCT02407236 PROgECT, NCT01988961.

Techniques: Microarray, Recombinant, Cell Recovery, Sequencing, Software

Journal: Dose-Response

Article Title: Ferroptosis-Associated Extracellular Matrix Remodeling in Radiation-Induced Lung Fibrosis Progression

doi: 10.1177/15593258241289829

Figure Lengend Snippet: Establishment of mouse RILF model and analysis of transcriptome microarray data analysis. (A) HE and masson staining of lung tissue collected at 20 weeks after IR (control, 17 Gy). Lungs treated to 17 Gy dose irradiation develop foci of fibrosis. Collagen: blue; cytoplasm/epithelia: pink. Scale bar = 20 μm. (B) The PCA plot of the distribution status of the two groups (C) Volcano plots displaying significantly differentially expressed genes. |log2FC| ≥ 0.5 and P < 0.05. (D) Heatmap displaying the expressions of the DEGs. Blue corresponds to lower gene expression and red to higher gene expression. (E) Bar plot of enriched KEGG pathways/GO terms showing DEGs.

Article Snippet: Gene expression was detected using a transcriptome microarray (Mouse Sentrix-6 V2 Whole Genome Bead Chip, BD-201-0202; Illumina, Inc., San Diego, CA, USA).

Techniques: Microarray, Staining, Control, Irradiation, Gene Expression